منابع مشابه
Cell wall residues in yeast protoplast preparations.
Protoplast preparations made from Saccharomyces cerevisiae by prolonged treatment with snail digestive juice contained fibrils and chitinous bud-scar residues from the original cell wall.
متن کاملEnhanced intraspecific protoplast fusion in yeast.
Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca2+. The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cer...
متن کاملImprovement of protoplast regeneration by growing yeast cells hypertonically.
The high osmotic pressure provided for in vitro transformation processes to support naked protoplasts of Saccharomyces cerevisiae was found to hurt regeneration of the protoplasts to walled cells. Preadaptation of the cells by growth under the same hypertonic conditions as those used for transformation doubled the rate of protoplast regeneration and in turn resulted in a higher yield of transfo...
متن کاملEffect of Potassium Ions on Protoplast Generation during Yeast Induction from Mucor circinelloides Tieghem
Mucor circinelloides aerobically exhibits coenocytic thallic growth habit with straight and circinate sporangiophores which culminate in globose or pyriform columellae enclosed within sporangial walls. It undergoes dimorphic switch with its conversion to multipolar budding yeast-like cells or thallic conidia. This paper confirms the induction of plurality of reproductive structures of the pleom...
متن کاملProtoplast cultures and protoplast fusion focused on Brassicaceae – a review
The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breeding. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resis...
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ژورنال
عنوان ژورنال: Nature
سال: 1967
ISSN: 0028-0836,1476-4687
DOI: 10.1038/216723c0